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ydiS [2020-04-11 17:33:25]
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ydiS [2020-04-11 17:33:25]

component of the BsuM DNA restriction system, similar to 5-methylcytosine-specific restriction enzyme
locus
BSU_06100
pI
8.70
mw
39.88 kDa
protein length
343 aa Sequence Blast
gene length
1032 bp Sequence Blast
function
BsuM DNA restriction
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.4|DNA restriction/ modification]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.5|Prophage 3]

    • Gene

    Coordinates
    660,596 661,627

    The protein

    Catalyzed reaction/ biological activity

  • Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates (according to UniProt)

    • Structure

  • [PDB|6HZ4] (5-methylcytosine-specific restriction enzyme from E. coli, corresponds to aa 29 ... 223, 29% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|4BD4CFB10700907DF1BBF3C9CFACAF573588E85F|ydiR]-[gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]-[gene|D36B91B3C8EAFEB80A7395C62A50A5F80B8CC5C5|ydjA]
    description
    [Pubmed|11751814]
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    Biological materials

    Mutant

  • MGNA-C208 (ydiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2206 NBRP B. subtilis, Japan]
  • DELTA0 (''ydiS trpC2''), available at BGSC
  • BKE06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA
  • BKK06100 ([gene|A8C606348C8C6FC3BB9786C06C9BFE9D3519A041|ydiS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06100 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAGTTCATTTCCCCTAT, downstream forward: _UP4_TCGTCTTTTGTGAGTGATTA

    • References

  • 11751814,22092861